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BMC Infectious Diseases Dec 2006There are few reports in the literature of invasive infection caused by Brevundimonas vesicularis in patients without immunosuppression or other predisposing factors....
BACKGROUND
There are few reports in the literature of invasive infection caused by Brevundimonas vesicularis in patients without immunosuppression or other predisposing factors. The choice of antimicrobial therapy for bacteremia caused by the pathogen requires more case experience to be determined.
CASE PRESENTATION
The case of a 40-year-old previously healthy man with subacute endocarditis proposed to be contributed from an occult dental abscess is described. The infection was found to be caused by B. vesicularis on blood culture results. The patient recovered without sequelae after treatment with ceftriaxone followed by subsequent ciprofloxacin therapy owing to an allergic reaction to ceftriaxone and treatment failure with ampicillin/sulbactam.
CONCLUSION
To our knowledge, this is the first report of B. vesicularis as a cause of infective endocarditis. According to an overview of the literature and our experience, we suggest that third-generation cephalosporins, piperacillin/tazobactam, and ciprofloxacin are effective in treating invasive B. vesicularis infections, while the efficacy of ampicillin-sulbactam needs further evaluation.
Topics: Adult; Anti-Bacterial Agents; Echocardiography, Transesophageal; Endocarditis, Bacterial; Follow-Up Studies; Humans; Male; Pseudomonas; Pseudomonas Infections; Rare Diseases; Risk Assessment; Severity of Illness Index; Treatment Outcome
PubMed: 17194310
DOI: 10.1186/1471-2334-6-179 -
Environmental Biosafety Research 2007Natural transformation is assumed to be the most likely mechanism by which DNA from transgenic plants could be horizontally transferred to bacteria. In order to...
Natural transformation is assumed to be the most likely mechanism by which DNA from transgenic plants could be horizontally transferred to bacteria. In order to determine the occurrence of naturally transformable bacteria amongst bulk and rhizosphere soil bacteria, different transformation strategies were employed using either plasmid DNA (IncQ plasmids pSM1890 and pSM1885, conferring GFP, Sm(r), Gm(r) and GFP, Sm(r), Tc(r), respectively) or genomic DNA from rhizosphere isolates, which were chromosomally tagged with mini-Tn5 (GFP, Tc(r)), as transforming DNA. Transformation assays were done in microtiter plates (262 isolates and pSM1890 or pSM1885), on filters (i) with rhizosphere bacterial community mixed with pSM1890 or pSM1885, (ii) with 24 rhizosphere or soil bacterial isolates mixed with genomic DNA of the corresponding mini-Tn5-tagged strains, and in the rhizosphere of tobacco plants inoculated with rifampicin-resistant bacterial isolates and genomic DNA of the corresponding mini-Tn5-tagged strains added. One transformant colony was obtained when Brevundimonas vesicularis was transformed with genomic DNA of the corresponding mini-Tn5-tagged strain. Attempts to reproduce this result were unsuccessful. With this single exception, transformants were neither detected in the collection of isolates nor in the rhizosphere bacterial community. Acinetobacter baylyi BD413 used as a positive control showed drastically reduced transformation frequencies with plasmid pSM1890 as transforming DNA when mixed with the rhizosphere pellet. All transformants were characterized by BOX-PCR fingerprints, and three different BOX patterns were revealed. Sequencing the 16S rRNA gene showed that all transformants could be assigned to Acinetobacter sp. Since transformants were only observed in the positive control, the introduced BD413 either underwent genomic rearrangements, or competence of the Acinetobacter population present in the rhizosphere was stimulated by the introduction of BD413. The various transformation assays performed indicate that the proportion of rhizosphere or bulk soil bacteria which are naturally transformable is negligibly low.
Topics: Blotting, Southern; Gene Transfer, Horizontal; Plant Roots; Plasmids; Polymerase Chain Reaction; Soil Microbiology; Transformation, Bacterial
PubMed: 17961483
DOI: 10.1051/ebr:2007035 -
Heliyon Mar 2024In water-stressed regions, treated acid mine drainage (AMD) water for irrigated agriculture is a potential solution to address freshwater scarcity. However, a...
Unlocking water potential in drylands: Quicklime and fly ash enhance soil microbiome structure, ecological networks and function in acid mine drainage water-irrigated agriculture.
In water-stressed regions, treated acid mine drainage (AMD) water for irrigated agriculture is a potential solution to address freshwater scarcity. However, a significant knowledge gap exists on the short and long-term effects of treated AMD water on soil health. This study used high-throughput Illumina sequencing and predictive metagenomic profiling to investigate the impact of untreated AMD (AMD), quicklime- (A1Q and A2Q) and quicklime and fly ash-treated AMD water (AFQ) irrigation on soil bacterial diversity, co-occurrence networks and function. Results showed that untreated AMD water significantly increased soil acidity, electrical conductivity (EC), sulfate (SO), and heavy metals (HM), including reduced microbial diversity, disrupted interaction networks, and functional capacity. pH, EC, Cu, and Pb were identified as key environmental factors shaping soil microbial diversity and structure. Predominantly, , , KB913035, , and , known for their adaptability to acidic conditions and metal resistance, were abundant in AMD soils. However, soils irrigated with treated AMD water exhibited significantly reduced acidity (pH > 6.5), HM and SO levels, with an enrichment of a balanced bacterial taxa associated with diverse functions related to soil health and agricultural productivity. These taxa included , , , , , , , , , and Moreover, treated AMD water contributed to higher connectivity and balance within soil bacterial co-occurrence networks compared to untreated AMD water. These results show that quicklime/fly ash treatments can help lessen impacts of AMD water on soil microbiome and health, suggesting its potential for irrigated agriculture in water-scarce regions.
PubMed: 38533070
DOI: 10.1016/j.heliyon.2024.e27985 -
3 Biotech Dec 2016Endophytes are microorganisms which live symbiotically with almost all varieties of plant and in turn helping the plant in a number of ways. Instead of satisfactory...
Endophytes are microorganisms which live symbiotically with almost all varieties of plant and in turn helping the plant in a number of ways. Instead of satisfactory surface sterilization approaches, repeatedly occurring bacterial growth on in vitro rootstock cultures of peach and pear was identified and isolated as endophytic bacteria in our present study. Five different isolates from peach rootstocks were molecularly identified by 16S rRNA gene sequencing as Brevundimonas diminuta, Leifsonia shinshuensis, Sphingomonas parapaucimobilis Brevundimonas vesicularis, Agrobacterium tumefaciens while two endophytic isolates of pear were identified as Pseudoxanthomonas mexicana, and Stenotrophomonas rhizophilia. Identified endophytes were also screened for their potential of plant growth promotion according to indoleacetic acid (IAA) production, nitrogen fixation, solubilization of phosphate and production of siderophore. All seven endophytic isolates have shown positive results for IAA, nitrogen fixation and phosphate solubilization tests. However, two out of seven isolates showed positive results for siderophore production. On the basis of these growth promoting competences, isolated endophytes can be presumed to have significant influence on the growth of host plants. Future studies required to determine the antimicrobial susceptibility profile and potential application of these isolates in biological control, microbial biofertilizers and degradative enzyme production.
PubMed: 28330195
DOI: 10.1007/s13205-016-0442-6 -
Case Reports in Infectious Diseases 2019Scombroid poisoning is a predominantly self-limited illness associated with ingestion of poorly handled fish. It is not frequently associated with bacteremia and has...
Scombroid poisoning is a predominantly self-limited illness associated with ingestion of poorly handled fish. It is not frequently associated with bacteremia and has never been described with septicemia. We describe a case of a man who presented in shock with histamine poisoning after ingesting sushi. Blood cultures grew an uncommon pathogen, . This case demonstrates systemic bacterial infection in the setting of histamine poisoning, which is an atypical presentation for a well-known foodborne illness.
PubMed: 31815024
DOI: 10.1155/2019/2148654 -
Shokuhin Eiseigaku Zasshi. Journal of... Aug 2008We evaluated the microbiological safety of bottled mineral water products commercially available in Japan. Of 10 bottled mineral water products manufactured in Japan, no...
We evaluated the microbiological safety of bottled mineral water products commercially available in Japan. Of 10 bottled mineral water products manufactured in Japan, no bacteria or fungi were detected in 9 (90%), but 1 (10%) contained 1.8x10(3) colony-forming units (cfu)/mL. Of 12 bottled mineral water products manufactured in the EU, 11 (91.7%) contained 23-3.5x10(4) cfu/mL. On the other hand, of 5 bottled mineral water products manufactured in North America, 2 (40%) contained 2.3x10(2)-2.5x10(3) cfu/mL. The detected microorganisms were glucose-nonfermentative Gram-negative bacilli such as Brevundimonas vesicularis, Moraxella spp., and Burkholderia cepacia, but Pseudomonas aeruginosa was not detected in any product. For immunocompromised host patients being managed in ultra-clean rooms, the examined bottled mineral water products manufactured in Japan, except 1, were microbiologically safe.
Topics: Gram-Negative Bacteria; Humans; Immunocompromised Host; Japan; Mineral Waters
PubMed: 18787316
DOI: 10.3358/shokueishi.49.308 -
International Journal of... 2008Brevundimonas vesicularis is a non-fermenting gram-negative bacillus, aerobic and motile. This microorganism is ubiquitous in the environment and has rarely been...
Brevundimonas vesicularis is a non-fermenting gram-negative bacillus, aerobic and motile. This microorganism is ubiquitous in the environment and has rarely been implicated in human infections. We present the second case of cutaneous infection caused by B. vesicularis in an immunocompetent patient.
Topics: Aged; Amoxicillin; Anti-Bacterial Agents; Caulobacteraceae; Gram-Negative Bacterial Infections; Humans; Male; Microbial Sensitivity Tests; Skin; Skin Diseases, Infectious
PubMed: 18547490
DOI: 10.1177/039463200802100226